When Luan overhears her siblings complaining about her comedy, she decides to give it up.
As the siblings go about their day, Luan arrives, and begins pulling pranks on them (such as hitting Lincoln with a boxing glove on a spring and shocking him with a joy buzzer, makeup related puns with Lori and Leni, burning Luna while showering by flushing the toilet, throwing a pie on Lisa, a knock-knock joke with Lucy using Edwin, a whoopee cushion with Lynn, a tofu-related joke with Lola, and an egg-related joke with Lana), while also announcing her upcoming comedy gig at a comedy club called The Chortle Portal. After pranking every one of her siblings, the siblings begin venting on how annoying Luan can be with her jokes. Unfortunately, Luan, who was heading downstairs, overhears them venting, and immediately becomes heartbroken, and sadly heads back up.
The next day, Luan begins acting normal towards her siblings, such as giving Lincoln a glass of punch, asking Lori and Leni for a hairbrush, supporting Lisa with her equations, asking Lynn and Lucy for a dollar (which they don't respond to), and asking Lola for a dollar (which she actually responds to). The remaining siblings are weirded out at Luan's new attitude, but eventually decide to celebrate by eating pie, since it means they can get a break from the pranks. Later, as Lincoln begins to collect everyone's trash, he discovers that Luan has thrown away her Chortle Portal flyers. When Lincoln informs Luan of this, she says that she threw them away on purpose, saying that she's giving up on comedy after hearing their venting the previous day. Lincoln tries to apologize, but Luan beings tossing out all of her props, even Mr. Coconuts. This causes Lincoln to break the news towards the remaining siblings, who are shocked, since Luan was so anticipated to partake in her gig at The Chortle Portal. As a result, they begin to devise a plan on how to get Luan into comedy again.
The next day, the siblings attempt make Luan get into comedy by setting up jokes so Luan can finish the punchline, but Luan just simply gives them rational/straightforward answers instead of making some kind of joke or punchline out of them. Realizing that plan didn't work, they go with a second plan, which involves making spit takes at anything Luan says, even if it's not a joke. Luan, getting fed up with her siblings after realizing their intentions, calls them out, saying that no matter how hard they try, she's not going to go back to comedy.
Later, Lisa has revealed that she has made a time machine to transport themselves to the past two minutes before Luan overheard them venting about her. But at the same time, Lincoln arrives with all of Luan's thrown away props, saying that he has a plan of his own. When Lisa says that side effects include having their butts on where their faces would be, the sisters decide to listen to Lincoln's plan. Lincoln tells his sisters his plan, and the sisters decide to go along with it.
At The Chortle Portal, the sisters have offered to let Luan come with them, saying that even if she's given up on comedy, she can still enjoy the presentation. The host announces that the first comedian to appear on stage is Lincoln, much to Luan's befuddlement. Lincoln appears onstage, holding one of Luan's comedy journals. As Lincoln begins to recite the jokes written in the journal, he suddenly starts screwing up on the punchlines. Luan, getting more and more stressed out at Lincoln's failures, rushes onstage, and corrects Lincoln's slip-ups. Suddenly, the audience begins laughing, finding Luan's humor funny. Pleased with the reception, Luan begins doing her gig, while Lincoln sits down with his sisters, happy that the plan worked.
The next day, as the siblings get their breakfast, Luan arrives, happy and content. The siblings congratulate her on a successful gig last night, and apologize for making her lose interest in her favorite thing. Luan forgives her siblings and promises to tone down her pranks. Suddenly, Luan trips over Lana, and tries to resist the urge to make jokes, but the siblings tell her that she can go ahead and crack a few. As Luan beings making breakfast related puns, the siblings leave, happy that Luan is back to doing what she loves most, while Luan couldn't be any happier to do what she likes.
This is the thirtieth episode where Lincoln does not speak to the viewers.
This is the second time Leni puts on makeup.
In this case, Leni does it correctly, unlike "Attention Deficit", in which she was still learning how to.
The trick were the Louds laugh at Luan despite not telling a joke to get her back is commonly used to stop comedians from delivering the punchline.
Even though she gave up comedy, Luan did not remove her gag flower from her outfit, which is used as a comedy prop.
Karma: The siblings complained about Luan's pranks and jokes, so she quit comedy, and became more serious with what she says.
Irony: Lincoln's poor performance got Luan to return to comedy, though it's obvious his intent was to put on a successful performance so Luan would see the audience laughing at HER jokes, and realize that she was funny.
After "Attention Deficit", because Leni can apply makeup, and in that episode she can't as she was only learning to.
After "Read Aloud", because Lola is able to read Luan's diary.
When the Louds attempt to make jokes so Luan can finish with a punchline, Lori and Leni say that its "wet" outside, possibly so Luan can say "That's what she said!", but Luan doesn't comply.
Possible: The way Lincoln gave Luan a warning before entering her room may give the implication that he once walked in on one of his sisters doing something he wasn't supposed to see while he was collecting the trash.
Don't overreact to criticisms and give up your interests; just keep practicing.
Just because other people don't believe in you, it doesn't mean you should lack faith in yourself too.
Accept people for who they are.
Always be true to who you are.
No Laughing Matter - The title is a reference to the term of the same name, which means something serious is something that should not be joked about.
Peanuts - The baby on Luan's invitation looks like Charlie Brown.
Regular Show - This episode has a similar premise to the Regular Show episode, "Prankless", as both episodes involve the character with a knack for pranking (Muscle Man and Luan) giving up on their passion out of pity, and the other characters trying to get them into pranking again.
Hey Arnold! - This episode too had a similar premise to the episode "Deconstructing Arnold", and in that episode, Arnold's classmates get really tired of Arnold telling them all to stop telling everyone to do the right thing all the time, in which it leads Arnold to stop giving them advice and leave them alone, and after Arnold's classmates realize Helga used them to give them her horrible advice, they all want to get Arnold back into telling them the right thing to do.
Coincidentally, the show stars Jessica DiCicco, the voice of Lynn and Lucy.
My Little Pony - Lola has a plush unicorn with a star on its flank, which is a reference to the design of the ponies from this popular toy brand.
Full House - The ending is the same as in the episode "But Seriously, Folks".
The Fresh Prince of Bel Air - Lincoln messing up at the comedy show until Luan does her routine is just like in the episode "Ain't No Business Like Show Business", when Will bombs a comedy routine until his comedian friend Keith saves the day.
SpongeBob SquarePants - The joke of Luna getting injured in the shower is similar to how Potty tried to get Patchy's attention by flushing the toilet in the half hour episode, The Sponge Who Could Fly.
When Luan is about to open Lucy's coffin to give her a flyer, her right arm is disconnected.
Lily has visible eyebrows during the sibling meeting, and when Lisa is explaining how her time machine works.
When Luan says "That's the wrong punchline!", the eyelashes on Lori's right eye are disconnected.
Luan's face was covered with soda after Leni did a spit take on her, but when she headed downstairs, her face is completely clean, despite not being shown to wipe the soda off of her face.
Lincoln's hair tuft disappears after Luan says she's "out of the comedy game", but when he runs over to her, it comes back.
When Luan says "Want some punch?", Lincoln's bags are missing.
In one scene, Lori's left eyelash is slightly curved.
When Leni flushes the toilet while Luna is in the shower, Lori's eyelashes are missing.
In the Latin American version, when Lola says "Toe-fu? Really?" (translated as "Toda la mano? En serio?"), she says it in Lynn's voice.
Luan cracking jokes.
The siblings trying to make Luan get into comedy again.
Which also leads to Luan not buying it.
The siblings doing spit takes in somebody's face (mostly Luan's face).
Someone hurting Luna accidentally by flushing the toilet when Luna was in the shower (happens twice).