Lana is a tomboy, contrasting with her twin's more girly personality. She loves to play in mud and enjoys getting her hands dirty. Her pleasures are found in filth of all kinds, and anything unhygienic, from frogs to digging in the trash for chewing gum to doing business outdoors. Despite her tomboyishness and gross habits, she is a decent enough person and is loving towards her family.
She is a junior expert plumber, this and other jobs close to filth, fixing artifacts, like in "Sleuth or Consequences", where she uses "Big Bertha" a plunger to unclog the toilet, although also aiming to make a poop big enough to clog the toilet herself. Other example being in "Suite and Sour" where she fixed a shower. She also possesses extensive knowledge in automotive repair and machinery, and sometimes can be seen fixing Lola's car or even Vanzilla.
"Lana Loud is the resident Ms. Fix-It, and is always ready to lend a hand, no matter how dirty the job. Need your toilet unclogged? Snake fed? No job is too tough, or too gross to take on. All she asks in return is a little A-B-C gum, or a handful of dog food."
Lana is nearly identical in her physical appearance to Lola. She has blonde hair, with two downward pigtails tied down with red scrunchies, and is missing her front teeth, like her twin does.
She wears a red baseball cap backwards with a small square hole, white sneakers with blue stripes, and a murky green t-shirt under dark blue overalls. Her nightwear just consists of a murky green shirt. Her swimsuit is a blue one piece, which looks like overalls. Her snow attire consists of a yellow coat with matching pants, blue boots with matching gloves, completed with green earmuffs.
Lana's hat is a red baseball cap that she wears in every episode she appears in. Lana never wears her hat forwards, she always wears it backwards, and Lana is also shown to sleep with her hat on. The only time where Lana did something that involved her hat was when she wanted Lincoln to sign it in "The Loudest Yard".
In an alternate universe where the sisters are boys, Lana is a boy named Leif. Similar to Lana, Leif is obsessed with mud, dirt, and animals. Unlike Lana, who is missing her front teeth, Leif is missing his canines.
However, he is much more abrasive than Lana, being mean towards his twin brother Lexx (Lola). He is also very harsh to Lincoln and plays keep-away with the time watch that he needs to go home.
However, in the alternate universe where it's one girl and ten boys (instead of one boy and ten girls), Leif's shown to be very caring and protective towards Linka Loud, Lincoln's female counterpart similar to Lana with Lincoln.
Leif eventually ceases to exist, as the entire adventure was all just a dream.
In the minicomic Deuces Wild!, Lana interprets the superheroine called Royal Flush, who is also one of Ace Savvy's sidekicks. She uses a pipe wrench to open up a fire hydrant. In the comic, she is summoned by Savvy (played by Lincoln) alongside the rest of Savvy's deck (played by the rest of her sisters) to help him and One-Eyed Jack (played by Clyde) to defeat the gas monster. After they defeat it, they prepared to fight against a garbage monster.
She appears in Lincoln and Clyde's comic in "Pulp Friction", where she uses her wrench to loosen the bolts on the shark tank, which prevents Wild Card Willy from escaping.
Lana doesn't know what a dress is, calling it a "weird, sparkly towel".
She dislikes the smell of hairspray, because she says that it smells like "princess farts".
She keeps a variety of tools with her, claiming that it is the "handyman's code".
She scratches her butt to help her think.
Lana is one of the most tomboyish of the Loud sisters. In fact, she's the second most tomboyish of her sisters after Lynn, due to her loving anything boyish. However, she seems to have a slight feminine side as she wears her hair in pigtails, wears a nightgown to bed, displays a very strong maternal attitude towards her pets, and squeals/giggles sometimes when happy or excited. Also, Lana doesn't seem to be as interested in playing sports as Lynn is.
When Lana grows up, she wants to be a "plumber-veterinarian-steam fitter".
Lana has some similarities to Wakko Warner from Animaniacs, such as a love for dirt, burping habits, and even the same backwards hat.
Her favorite things to eat are boogers and ABC gum.
According to Chris Savino, he states that the sisters who are girly-girls or fashionistas are usually seen with three eyelashes (Leni, Lola, and Lily). Lana, despite having three eyelashes, is a tomboy instead. This is because Lana and Lola are identical twins.
Lana is the second sister after Lori to get an entire episode with just her and Lincoln.
In "Frog Wild", it is revealed how she met Hops, and how he became both her pet, and her friend.